Endocrinology and Metabolism Research Institute

Tehran University of Medical Sciences


2/29/2016 | Creator: 130

Authors: Hosseini M1, Mehrabi F1, Ganjali MR2,3, Norouzi P2,3.

Article URL:;jsessionid=F1C0D9053191B10BB8473633E3CF4C26.f02t04

Affiliation: Endocrinology and Metabolism Research Institute

Description: A fluorescent aptasensor for detection of oxytetracycline (OTC) was presented based on fluorescence quenching of DNA aptamer-templated silver nanoclusters (AgNCs).

The specific DNA scaffolds with two different nucleotides fragments were used: one was enriched with a cytosine sequence fragment (C12) that could produce DNA–AgNCs via a chemical reduction method, and another was the OTC aptamer fragment that could selectively bind to the OTC antibiotic.

Thus, the as-prepared AgNCs could exhibit quenched fluorescence after binding to the target OTC. The fluorescence ratio of the DNA–AgNCs was quenched in a linearly proportional manner to the concentration of the target in the range of 0.5 nM to 100 nM with a detection limit of 0.1 nM.

This proposed nanobiosensor was demonstrated to be sensitive, selective, and simple, introducing a viable alternative for rapid determination of toxin OTC in honey and water samples.

Journal Name: Luminescence. 2016 Feb 22. doi: 10.1002/bio.3112
Pub. Year: 2/22/2016
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