Biosensor Research Laboratory
Health care is the main area of biosensors applications in recent years. Measurements of analytes in the biological samples are regularly needed to show a patient’s metabolic state especially for those in hospital, and even more so if they are in intensive care. Many of these substrates have been determined by samples of urine and blood being taken away to a medical analytical laboratory for classical analysis, which may not be complete for hours or even days.
In emergency cases, an ideal alternative could be on-site biosensors and sensors, a kind of devices, which does not depend on instrumentation and is also rapid, sensitive, specific, cheap, and easy to interpret.
The use of on-the-spot biosensors and sensors enable results to be obtained in minutes at most. Therefore our research focus is to develop biosensor and nano-biosensors for rapid and on-site detection of analyte in biological fluids What is a BIOSENSOR? A biosensor is a device that incorporates a biological recognition (sensing) element in close proximity or integrated with the signal transducer, to give a reagent less sensing system specific to a target compound (analyte).
Biosensor technology encompasses a wide range of disciplines due to the variety of biological recognition elements and signal transducers used. Typical biological recognition elements used in biosensors range from antibodies, enzyme, affinity ligand (e.g., lectin), receptor, peptide, oligonucleotide, organelle, organism, or tissue slice enzymes. The methods of signal transduction used range from electrical, optical, electrochemical, thermometric, piezoelectric and magnetic. The research activity at the Biosensors laboratory includes a wide range of projects.
The primary goal of our laboratories is to understand biological processes at the molecular level, with the ultimate aim of using this knowledge to tackle specific problems in human health and disease.
The following technical methods have been used in our research Labs:
1- Antibody engineering (Phage display technology). Production of gene and cDNA library and phage display techniques including panning and selection of library.
2 - Immunochemistry and immunoassay methods including ELISA, RIA, EIA,Immunochromatography, Immunocytochemistry.
3 - Protein purification methods and Chromatography including sieve, ion exchange, affinity,…
4 - Electrophoresis including PAGE, SDS-PAGE, …
5 - Blotting methods including Western blot.
6 - Cell culture techniques.
7 - Hybridoma technology including cell fusion, hybridoma selection and limiting dilution.
8 - Molecular biology techniques including DNA and RNA purification, PCR, RT-PCR, DNA electrophoresis, cloning.
9 -Chemical conjugation of molecules.
10 - Gene expression and over expression methods in bacteria, yeast and eukaryotic cells.
11- Nanoparticle preparation and conjugation of protein to them